MSDial crashes after closing Table viewer

April 19, 2021, 01:28:49 AM

Dear all,

at the moment I have small sets of samples (around 100 per experiment).

The problem I am facing with all my different experiments is after I close the Table viewer for the aligned chromatograms, the program crashes about 20% of the time. I have the feeling, the longer I am in the table viewer, the higher the chances are.

Is there a logfile or a debugging mode I can view/switch on?

Thank you,
all the best,

Martin

Language: English (UK)
Windows 10 Pro

MS-DIAL ver. 4.60

#Data type
Data type   Centroid
Ion mode   Positive
Accuracy type   IsNominal
#Data collection parameters
Retention time begin   0
Retention time end   45
Mass range begin   50
Mass range end   600

#Data processing
Number of threads   4

#Peak detection parameters
Smoothing method   LinearWeightedMovingAverage
Smoothing level   3
Average peak width   20
Minimum peak height   1000
Mass slice width   0.5
Mass accuracy   0.5

#MS1Dec parameters
Sigma window value   0.5
Amplitude cut off   10

#identification
MSP file   F:\Biology\Libraries\GCMS DB_FiehnBinbase-KovatRI-VS2.msp
RI index file   see last
Retention type   RI
RI compound   Alkanes
Retention time tolerance   0.5
Retention index tolerance   25
EI similarity library tolerance   70
Identification score cut off   70
Use retention information for scoring   True
Use retention information for filtering   False
Use quant masses defined in MSP format file   True

#Alignment parameters setting
Reference file   F:\Biology\Data\2021_Macro_ExpII_IntSt-MB\BT TOF\mzml\abf\Pool_1_b1_SL_start_275sec.abf
Retention time   RI
Retention index tolerance   20
Retention time tolerance   0.075
EI similarity tolerance   70
Retention time factor   0.5
EI similarity factor   0.5
Identification after alignment   False
Gap filling by compulsion   True
Basepeak mz selected as the representative quant mass   False

#Filtering setting
Peak count filter   0
N% detected in at least one group   0
Remove feature based on peak height fold-change   True
Sample max / blank average   5
Sample average / blank average   5
Keep identified and annotated metabolites   True
Keep removable features and assign the tag for checking   True

#RI dictionary (analysis file path / ri dictionary file path)
cropped

Re: MSDial crashes after closing Table viewer

Reply #1 – April 21, 2021, 01:09:27 AM

Dear Martin,
In my experience, if I am editing the alignment table (see picture below), and at the same time press Ctrl + D to change the feature to unknown, the program will crash. It happens all the time. So, to change the feature to Unknown in the alignment table, we have to make sure that we are not editing the table.

I don't know if this is your situation. Hope it is helpful for you.

Best,
Sukis

Re: MSDial crashes after closing Table viewer

Reply #2 – April 21, 2021, 01:36:06 AM

Dear Skuis,

thank you for your reply!

Maybe the problems are somehow connected that you have it while editing the alignment table, and I have it in the table viewer.

Does the crash also mostly/always happen when you close the window?

All the best,
Martin

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