Normalization using cell protein content April 20, 2021, 08:25:02 AM Dear Community,I am working on a cell-based metabolomic project. Does anyone know how to normalize the data using protein content in MS-DIAL?Thank you!Pan Quote Selected
Re: Normalization using cell protein content Reply #1 – April 24, 2021, 12:58:59 PM Hello Pan,do you have additionally an internal standard in your samples?If yes: IS normalization in MS Dial, then take the alignment sheet and divide your samples by the corresponding protein amount.If not: take the alignment sheet and divide your samples by the corresponding protein amountYou can do a TIC / mTIC normalization, but I'm not very confinced it has a lot of benifits.All the best,Martin Quote Selected
Re: Normalization using cell protein content Reply #2 – April 24, 2021, 03:22:08 PM Thanks Martin!Yes, I have an internal standard, will do as you suggested.Best,Pan Quote Selected
Topic: Normalization using cell protein content