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Topic: MS DIAL - Scoring issues (Read 4253 times) previous topic - next topic

MS DIAL - Scoring issues


I have a little issue to understand how the scoring in MS DIAL work. I use my own spectrum in high resolution as reference, and compare it to samples in lower resolution.
I don’t understand why the CAR(16:0) do not match the reference despite having good score both in dot product and reverse product, and a total score above my cut of at 50%. What does the « presense » in library information represent?

Thank you,


Re: MS DIAL - Scoring issues

Reply #1
Try to check the option of "Use RT for filtering candidates"  and "Use RT for scoring candidates". If you use these options, please check your RT tolerance setting. The total score calculated in the compound search windows does not consider the RT score, but in the data processing procedure, the RT score is also considered to calculate the total.