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Topic: Why does selection of an MSP-File alters the number of aligned features? (Read 340 times) previous topic - next topic

Why does selection of an MSP-File alters the number of aligned features?

Hi,

after processing the current data set I found out that it can make a difference for alignment, if you choose an msp file in the identificaton step vs. if you don´t.

I tested this for the current dataset in positive mode. By selecting an msp-file (the open-source file of MS-Dial) I got 14464 features. When no msp file was selected, I only got 14106 features after processing, although the same parameters were used. After all the difference in features is about 2%.

At the moment, I can not explain, how this can happen because the alignment should work the same whether you select an msp-file or not, isn´t it?

Best regards
FalcoB