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Topic: Strategies for resuspending in small volume after dry-down (Read 370 times) previous topic - next topic

Strategies for resuspending in small volume after dry-down

I am quite new to lipidomic sample prep, and I am wondering what strategies people use to get complete resuspension of their sample after drying down from a large volume.

An MTBE extraction protocol I'm looking at dries down 14 mL of solvent in a glass tube and would be resuspended in ~400 mL. Obviously internal standards can help control for losses, but what other tricks/techniques do you use to ensure the best recovery possible in this situation?

Thanks!

Re: Strategies for resuspending in small volume after dry-down

Reply #1
to your initial sample add a small volume of glycerol or some other viscous and non-evaporating liquid (non interfering with your measurement method ;). during drying analytes will be concentrated in the non-evaporated drop then re-suspend as needed