Strategies for resuspending in small volume after dry-down December 09, 2021, 01:57:19 PM I am quite new to lipidomic sample prep, and I am wondering what strategies people use to get complete resuspension of their sample after drying down from a large volume. An MTBE extraction protocol I'm looking at dries down 14 mL of solvent in a glass tube and would be resuspended in ~400 mL. Obviously internal standards can help control for losses, but what other tricks/techniques do you use to ensure the best recovery possible in this situation?Thanks!
Re: Strategies for resuspending in small volume after dry-down Reply #1 – December 09, 2021, 11:36:15 PM to your initial sample add a small volume of glycerol or some other viscous and non-evaporating liquid (non interfering with your measurement method . during drying analytes will be concentrated in the non-evaporated drop then re-suspend as needed