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Topic: No peak information (Read 4562 times) previous topic - next topic

No peak information

I have been trying to start using MS-DIAL to perform a metabolomics study using the negative ion mode. I have tried setting different parameters and uploading different files, however, I always get the same message: "There is no peak information for peak alignment. Please check your ion mode setting", then another message: "There is no peak information in (sample name). So please select other files or check ion mode for it and re-analyze it with the ion mode setting". What should I do?

Re: No peak information

Reply #1
Hi MaiAmmar,

If you're using .wiff or .wiff2 files for this analysis and running multiple samples/blanks in one project, you need to have the .wiff.scans of those files in the same folder so that MS-DIAL can read them. If you don't have the .wiff.scans, you can run single samples in multiple projects and you will still get peak IDs, if not any alignments with QCs or Standards.

Re: No peak information

Reply #2
Hi MaiAmmar,

I am running GC-MS data, but I have a similar issue. MSDIAL crashes during the alignment step because of no peak information.
The weird thing is that not all files have this problem and when I run different times, different files have this problem.