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Topic: MS1 tolerance in peak detection vs. alignment / BPC mass slice width (Read 2196 times) previous topic - next topic

MS1 tolerance in peak detection vs. alignment / BPC mass slice width

Hi,

I was wondering how to deal with the different setting options for the MS1 tolerance (in Da) for peak detection vs. alignment. Is it generally recommended to have a "looser" setting for peak detection and a correspondingly "stricter" setting for alignment, or should both parameters be set very similarly or even identically?

Moreover, I have a question regarding the function of the BPC mass slice parameter. Does it determines whether data points are within the same slice and then assigned to the same peak?
We work with a high-resolution MS (QTOF). Values like 0.1 Da or 0.05 Da as recommended by MS-Dial seem quite generous at first glance. But I may not have fully understood the meaning of this parameter.

Thanks for your input.
FalcoB