How to calculate p-value for a PLS-DA plot? June 27, 2022, 05:17:55 PM Hi, I have used the Metabolomics data to generate a PLS-DA plot using metaboanalyst. To be sure about the significant difference between two visibly different groups, I want to generate a p-value using some test (like t-test) for this multivariate data. Can you please guide me what test should I use or how to calculate the p-value?Best.