Skip to main content
Topic: S/N (signal-to-noise) filter in MS-DIAL (Read 137 times) previous topic - next topic

S/N (signal-to-noise) filter in MS-DIAL

Hi all,
I couldn't find an answer for this in this forum or generally online.

MS-DIAL routinely calls peaks like those in the attached screenshot. The average S/N of these peaks is extremely low. I would like to avoid these peak calls altogether: is there a setting in MS-DIALs peak picking/featurization that would allow me to avoid these?
If not, can I filter them out after peak-picking? Is there any place to set a threshold such that any feature with average SN below that threshold is not shown in the alignment?

I can certainly export the data to python and do it there easily, but for quick GUI based processing I would love to be able to do this.

Any advice greatly appreciated.

Thanks,
Will

Re: S/N (signal-to-noise) filter in MS-DIAL

Reply #1
Hi all,
I couldn't find an answer for this in this forum or generally online.

MS-DIAL routinely calls peaks like those in the attached screenshot. The average S/N of these peaks is extremely low. I would like to avoid these peak calls altogether: is there a setting in MS-DIALs peak picking/featurization that would allow me to avoid these?
If not, can I filter them out after peak-picking? Is there any place to set a threshold such that any feature with average SN below that threshold is not shown in the alignment?

I can certainly export the data to python and do it there easily, but for quick GUI based processing I would love to be able to do this.

Any advice greatly appreciated.

Thanks,
Will


Hi Will,

I suppose changing the amplitude under peak detection and MSDec tabs would get you the desired results? Does setting a higher cut-off not resolve this issue?

Are these so called peaks above your set noise level?

Re: S/N (signal-to-noise) filter in MS-DIAL

Reply #2
Yes, agreed, that a S/N filter in the "alignment table" during display of results in the GUI would help the analysis and visualization as well!