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Topic: Problems with Lipidomics mode: No peaks/zero ref matches (Read 2565 times) previous topic - next topic

Problems with Lipidomics mode: No peaks/zero ref matches

Dear Community,

I am trying to run my sample in the lipidomics mode but I got zero matches. It showed no peaks matched with reference libraries. Could you please suggest what could be the problem and how to solve this?

Thank you very much.

Re: Problems with Lipidomics mode: No peaks/zero ref matches

Reply #1
Hi Tomiwa Oyedokun,

Please check the following things and very likely:
1. Ensure that your LC-MS instrument acquired the data in MS/MS
2. While converting the data you are converting in a way that MS2 level data is retained and not lost.
3. More info on the instrument used and file converted used and sharing a downloadable link to the file help.
4. Another culprit could be too stringent filters set for RT matches (put it to 100 mins) or MS1 resolution could be impacting analysis.

Thanks,
Biswa

Re: Problems with Lipidomics mode: No peaks/zero ref matches

Reply #2
Thank you very much, Dr. Biswa for your response. I appreciate it.

To be more clear on my previous question. Actually, it's not that MS DIAL did not detect any hits. It did detect over 28,000 hits but after I compared the hits with the reference library, it did not display any peaks with the reference spectra. The hits were reduced to zero. Please see the two screenshots showing hits generated, and zero hits after I compared them to the reference library.

And as for the file conversion used, we used ABF converter: https://www.reifycs.com/AbfConverter/

Thank you so much once again.
Tomiwa Oyedokun

Re: Problems with Lipidomics mode: No peaks/zero ref matches

Reply #3
Actually, I'm seeing something similar when processing Thermo .raw data using the latest 4.9.221218 version. I see severely under-annotated results. When processing the data using v. 4.90 I do not see this problem. Please try to process your data using v. 4.90.
Also, I do not see this problem when processing Sciex data.
I hope this helps.
Vladimir.

Re: Problems with Lipidomics mode: No peaks/zero ref matches

Reply #4
Thank you everyone for your responses. I tried all your suggestions and other advice I found online but none seemed to work. I am still trying to find solution to that particular data while continuing with other tissue samples. And I will let you know when I am able to figure it out.

Thank you all,
Tomiwa Oyedokun