RT correction March 27, 2023, 03:47:06 AM Has anyone used RT correction successfully? I can see that it works to align the shifted peaks from my two batches of analysis (see RT_correction screenshot) but the peaks are distorted. See other pics for examples. Won't this affect quantification? I'm running v4.9.221218Thanks, Chris Last Edit: March 27, 2023, 03:51:18 AM by drchrispook
Re: RT correction Reply #1 – March 28, 2023, 12:47:07 AM Yeah, I don't like this. Instead I've worked out that converting your data to mzML means you can just add an RT shift to the time value of each scan. I've just done a fixed shift but if I spend a couple more hours sampling different shifts between my batches I could use a function to correct it instead.
Re: RT correction Reply #2 – April 12, 2023, 07:04:27 AM Quote from: drchrispook on March 28, 2023, 12:47:07 AMYeah, I don't like this. Instead I've worked out that converting your data to mzML means you can just add an RT shift to the time value of each scan. I've just done a fixed shift but if I spend a couple more hours sampling different shifts between my batches I could use a function to correct it instead. Please tell me more about this, is this done in ProteoWizard? I follow your blog and have benefitted when converting .L to .msp and merging them, so thank you.
Topic: RT correction