identification doesn't work in MSDIAL

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identification doesn't work in MSDIAL

June 22, 2023, 09:07:43 AM

Hi
Im using MSDIAL ver 4.9. Usually lipidomics work very well but now in metabolomics it doesn't make any identification. Im using the latest msp files from negative database in the MSDIAL repository.
Even we tried with the demonstration files and didnt work.
What would be a solution?

Thanks in advance,

Re: identification doesn't work in MSDIAL

Reply #1 – June 24, 2023, 08:22:24 AM

Hello,

Please share your Parameters.text file here after exporting it from your completed analysis!
I could give a look and see any obvious challenges.

Thanks,
Biswa

Re: identification doesn't work in MSDIAL

Reply #2 – June 29, 2023, 08:36:23 AM

Hi Biswapriya
There is the parameters for the example in CompMS (Hydrophilic metabolome profiling of Wine). I dont get reference matched nor suggested anotations.
Also I tried with my data with the spectral kits from CompMS and none of them worked (no refereced matched or suggested), I think there is a problem with MSDIAL in database loading or database format.

Thanks in advance