Proper Conversion for Waters .raw Files

October 23, 2023, 09:28:44 AM

Hi everyone,

I am trying to use MS-DIAL to analyze MS/MS files from a Waters Xevo QQQ instrument, and having difficulties converting the files. I am able to use the software perfectly fine with my Agilent .d files, but the converted .raw files always yield the same error message: No peaks detected. Check polarity settings. Despite the files being converted to .abf, they are not properly being read by MS-DIAL. From what I've seen online and in other posts, I believe the files might be converted incorrectly (they are below 9KB). I have tried converting them to mzML files as well, with the same result. Has anyone else had this issue before?

Re: Proper Conversion for Waters .raw Files

Reply #1 – December 13, 2023, 10:09:58 PM

Hello,
Please try this nice tool to convert to .mzML from Waters and then MSDIAL accepts mzML!
https://github.com/AnP311/Waters2mzML

Thanks,
Biswa