MS-DIAL error. Check polarity setting?

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MS-DIAL error. Check polarity setting?

December 07, 2023, 05:42:26 PM

I have LC-MS（Shimadzu）lcd. files and need to analyse with MS-DIAL. I followed below steps and at the end I get a message "No peak information. Check your polarity setting."

1. Converted .lcd files to .mzML using ProteoWizard

2. Converted .mzML files to .abf using Reifycs Analysis Base File Converter

3. imported .abf files to MS-DIAL and ran with below settings:

4. MS1 Data type (Centroid data)

5. MS/MS (Centroid)

6. Ion mode (Negative)

7. Metabolomics

8. Libraries loaded (MSMS-Neg-Vaniya-Fiehn_Natural_Products_Library_20200109.msp / BioMSMS-Neg-PlaSMA.msp / MSMS-Neg-FiehnHILIC.msp)

9. Get below error
"No peak information. Check your polarity setting."

Re: MS-DIAL error. Check polarity setting?

Reply #1 – December 13, 2023, 10:03:39 PM

Hello,

Please share the a) Parameters.text file that you can save after the analysis is concluded.
b) A link to a RAW file loaded onto Google drive or dropbox will also help me troubleshoot the issue.

Thanks,
Biswa

Re: MS-DIAL error. Check polarity setting?

Reply #2 – December 14, 2023, 03:06:35 AM

Dear Biswapriya
I’ve emailed you. Thank you in advance for offering your help 🙏