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Topic: Alignment between MS1 and MS2 files (Read 2525 times) previous topic - next topic

Alignment between MS1 and MS2 files

Hi everyone,
I have recently started to use MS-Dial on untargeted LC-MS/MS. I come to appreciate that it is a powerful and flexible system. And the tutorials that were put together by the developer and people in this community has been immensely helpful.

My questions is on alignment. In my study, MS1 data was acquired from all samples, and MS2 was only acquired on pooled QC samples to boost sensitivity. It doesn't seem that MS-Dial can align between MS1 and MS2 files, so as a result all the aligned peaks are missing MS2 identification. I'm curious if there is a way to align MS1 and MS2 files on MS-Dial. I would love to hear your thoughts if any of you have experience with similar issue.

Thank you!