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Topic: Loss of sensitivity (Read 5743 times) previous topic - next topic

Loss of sensitivity

Hello,

I'm currently working on a Waters Synapt (ESI + source) and I encounter an important loss of sensitivity over time, probably due to the source clogging (I'm working with intact proteins).
Which normalisation method would you recommend to fix this quasilinear "drift" ? Could we assume that this drift is similar to a dilution effect and correct it using PQN or Median normalisation ?

Thanks for your help.