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Topic: pSRM with Q-exactive (Read 5324 times) previous topic - next topic

pSRM with Q-exactive

Dear Sir,
 I am a PhD student at Heidelberg, and currently I am working on nLC-Exactive to quantify the sphingosine bases.
My questions is while its very simple to create a MRM method with the Q-trap instruments, I dont know how establish this method with Q-E,
          Mass transition
C17Sph 286/268 7.2
Sph 300/282 7.5
DHSph 302/284 7.6
S1P 380/264 8.7
DHS1P 382/284 9.0
C17S1P 366/250 8.2

Should I choose Targeted - SIM or data dependent?
I would appreciate if someone could share his/her experiences.

with my best regards.