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Topic: DataDependentScan analysis - screening for fragment ion (Read 5466 times) previous topic - next topic

DataDependentScan analysis - screening for fragment ion

Dear All,
I have one emerging question regarding analysis of DDA raw files done with Orbitrap XL.
I have found one interesting fragment ion (not precursor, just one fragment), that repeats in many MS2 spectras along all retention time. I wish to screen  whole raw file in order to look for that fragment, as it is probably related to one metabolite-marker in my study.
I have MassFrontier 7.0, Sieve and XCalibur - which of them  would be the most suitable, and how to do it? Which option to use?
I was desperately looking for some on-line manual videos, or any step-by-step explanations, however could not find any.

I would be very appreciated for any help.
thank you
Marynka