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Topic: Metabolite identification problem (Found 0 total...) (Read 4424 times) previous topic - next topic

Metabolite identification problem (Found 0 total...)

Dear All,

Hello, my name is Jonghyun Kim and I’m writing to ask about the XCMS online.

I want to identify metabolites in my extracts with XCMS online, but I was faced with a problem to get ID result. Every time when I submit Jobs, I could never find pvalue column from result table page. Only thing that I got was information of features and ‘Found 0 total, MS^2 spectra to match’.

Currently, I use Agilent 6520 Q-ToF and MassHunter Workstation Software B.05.00 (Build 5.0.519.0) for acquisition. I uploaded .d raw file (profile & centroid), mzData (centroid) file and mzXML file with 50 min gradient using JAVA. But all of them failed to give the ID result.

It would be greatly appreciated if you would provide some advice for successful identification.

https://xcmsonline.scripps.edu/viewlog. ... ne_log.txt

This is a log of my Single Job.

Thank you for taking the time to consider my problem.

Sincerely,

Jonghyun Kim