peak splitting: I want less peaks. October 05, 2023, 05:59:03 AM Hello, I'd like to ask for help. I hope there is an easy answer. My lipidomics abf files are quite big by themself (1+ Gb from QToF MSe), and the alignment is also very rich (13K features). Many of them come from peaks being split or picked as separate entities. In example attached below, there are four peaks. In some cases they have equal areas in some samples and in another subset of samples values can be very low or very high. Inspecting raw data on MassLynx show that they are in fact a single analyte. http://www.metabolomics-forum.com/index.php?action=dlattach;sa=tmpattach;attach=post_tmp_16020_eaa3aec5bf9a284059c7925ca1df2a57;topic=0Is there a setting a can play with to make sure the alignment will produce one instead of many features? I have tried changing smoothing, but without much success. Min. height = 1000. Please help,- Mariusz Quote Selected