Missing values in Lowess/Spline normalization method September 09, 2022, 08:23:13 AM Hello everyone,How should missing values have to be represented before using LOWESS/Spline normalization software? They should be represented with zeros "0", "NA", or only blank cells.Or do you recommended firstly doing missing values imputation and then doing LOWESS/Spline normalization for batch/signal correction?Thank you in advance for your answers. Quote Selected