Hi all. This may be a fairly elementary question, but I am a relative newcomer to the nuts-and-bolts of quantifying MS data. The data that my lab collects is centroided before analysis. Since each data point is an intensity at a discrete m/z and not a continuous trace like you get with profile collection, how are the shape of peaks reconstructed before peak detection? Is this at all related to the profStep argument supplied to the xcmsRaw function?
