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81
MS-DIAL / Re: Which version is more stable?
Last post by James -
I have heard that version 4.24 works for some people with these issues, but it doesn't fix the problems for me.

I don't do lipidomics, but I've found the most reliable way for me to avoid crashes is to first run alignment steps without any identification, and then go back and run identification after saving the alignment file. For some reason it seems to crash less frequently this way for me.

James
84
MS-DIAL / Which version is more stable?
Last post by MatyashSupp -
Hello everyone

 Im having alot of trouble with the new versions of MS Dial, even with 32gb ram I cant use it without trouble. Suffering with stutters and alot of crashes.

 Im running lipidomics with a single database with .wiff files and Dial crashes everytime, or just freeze.

Anyone know some version more stable?
86
MS-DIAL / Re: Peak integration in MS-Dial
Last post by MatyashSupp -
Hello Sergei
Normally, in my case, I just manually integrate it to zero.
I dont know if there's some way to put some 0 baseline, maybe at the normalization... IDK.
87
MS-DIAL / Re: Peak integration in MS-Dial
Last post by fourkin -
Hi JMS,

sorry for late reply: when I have ambiguous identification I check my standard panel, which I run along with unknown samples. This gives me RT info. Also, if ms2 spectra was acquired, I check it against the database (mzCloud for instance). Hope this helps.

Thanks.
Sergei.
88
MS-DIAL / Re: SCIEX 5600 Qtof data (IDA mode) with MSDIAL
Last post by MatyashSupp -
Hello Mwang, I got the same Sciex but I'm doing Lipidomics instead. But I did some tests to understand how MS DIAL works and I have some issues when, first: MS DIAL just dissapear with the channels, with makes me question about the confidence of DIAL, and second: I could just use my datas in MS DIAL when it accepts the .wiff, it because when I use ABF, I lost many data infos.

 Did u already tried to use the recent versions of DIAL? Maybe it'll help.
89
MS-DIAL / number of features depends on spectral library
Last post by fran999 -
I am using two spectral libraries to annotate HPLC-MS DDA data, with the default MSDIAL parameters, and I see that the two resulting feature tables have a different number of features despite using the same spectral dataset as input.
The sample types (samples, QCs and blanks) were provided, and the option "Remove features based on blank information" was not checked.

Is there any way to avoid MSDIAL to filter features out? I would like to keep the same number of features to compare the annotations obtained with the 2 libraries.
90
MS-DIAL / Large TGs identification/ spectrum prediction
Last post by vso -
Hello,

I wasn't sure where to post this but since lipidblast is offered with MS-DIAL I'll try here.

Is there a way to predict ms/ms spectra for structures that are not currently covered by lipidblast (e.g. TGs > 80 carbon atoms with unsaturated acyl chains) in order to be able to identify very large lipids. E.g. I have ms/ms spectra for TGs with precursors >1300Da that cannot be identified by MS-Dial.

Thanks in advance