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XCMS Online / no features detected
it is the first time that I use the XCMS software and I'm having troubles in carrying on the analysis. I've many chromatograms which have been converted from "raw" to the "mzXML" and then uploaded. The experiment is setted with 4 concentrations (0, 300, 600 and 900 micromolar) and 4 replicates for each concentartion. I've done a different dataset for each concentration.
Successively I've tried to do a "Pairwise job". I've compared the dataset "0" with the dataset "300" (it was working), but when I've tried to do a new "pairwise job" (0 vs 600 and 0 vs 900) I've got this error "There were no features detected in at least one of your samples. Please check feature detection settings and make sure the selected method is applicable for your data". What am I doing wrong? How can I solve this problem? I've got the same answer also when I've tried to do a "Multigroup job". Could somebody help me? Thanks in advance.