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MS-DIAL / MSDIAL Beginner
I'm just starting out with using MSDIAL and have a few initial questions. Would be great if anyone was able to help out with all or any of these please I am working with metabolomics data acquired from drosophila samples. My questions are:
1. In terms of selecting a library to use, I've had a look at the MSDIAL website and the downloads available on MONA at https://mona.fiehnlab.ucdavis.edu/downloads ,but I'm unclear on how to go about selecting one. For e.g. are they all suitable for all sample types or organism specific?
2. I understand that I also need to upload a library text file under the identification tab. I received an excel file with retention times from the facility that carried out the data acquisition. I'm wondering if this is what I would need to attach here (in text format) or if it's something else I would need to upload?
3. Finally, when I come to the section where I input samples to process: from the files sent to me by the facility that acquired the data I don't think I have anything that can be input as a standard in the class ID column when adding all my samples. Is it okay only to be entering pooled, QC and sample info ( and not any standards) or am I missing something?
If anyone can offer me any advice re the above it would be a massive help to me. Thanks