MAVEN January 09, 2019, 07:50:21 AM Dear Metabolomicians!Does anyone of you use MAVEN?I am having problems visualizing data. I ran an untargeted experiment with data containing MS1 and MS2 (Top10) spectra (QE Thermo Fisher). I converted the .raw files to .mzXML and opened it in MAVEN.In the attached screenshot you see the dashed TIC (why can't I get it smaller? How can I change the appearance?) and the EIC for the positively charged glutamate ion. Believe me, in Compound Discoverer or the Qualbrowser glutamate gives a strong and beautiful peak in the same dataset.Do you have a hint what I should be looking for? Or does MAVEN simply not work with untargeted data from MS1+2 Top10 experiments?Best,Debbie.[attachment deleted by admin] Quote Selected
Re: MAVEN Reply #1 – January 14, 2019, 08:20:03 PM Hi Debbie,I haven't used MAVEN and by the lack of responses, it doesn't seem like many (any?) people here use it.I would suggest trying a few things and letting us know if any worked/didn't:Increase the ppm window until you see the peak you expect. If you never see it, there is likely a bigger issue going on.Use msconvert and save the data with and without centroiding.Use msconvert and save the data with a filter for MS1 only.I assume you are using MAVEN for the analysis part of the program? Quote Selected
Re: MAVEN Reply #2 – January 15, 2019, 07:31:28 PM As another follow up, you could look at "El MAVEN" (https://elucidatainc.github.io/ElMaven/). The website says the following: QuoteMaven and El-MAVEN share following features:Multi-file chromatographic alignerPeak-feature detectorIsotope and adduct calculatorFormula predictorPathway visualizerIsotopic flux animatorEl-MAVEN is robust, faster and with more user friendly features compared to Maven.It is being updated fairly recently, with the latest release coming out just 5 days ago. Quote Selected