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Topics
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Topics - trammells
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XCMS / Heat Map Visualization
I have been attempting to visualize the data as a heat map but haven't found much luck in doing so. Ideally, I'd love to display the data showing changes in abundance with color between two conditions compared to a control group. Does anyone know the correct R code to perform this action? I have looked for the code to make a heat map but I really need something more similar to how microarray data is displayed.
Thank you for your input and if you require further information, please do not hesitate to ask,
Sam
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XCMS Online / Analytical versus Biological Replicates
I have a statistical question. I have three biological replicates and for each biological replicates two injections (2 analytical replicates). When performing an analysis, I see no way to differentiate between the two types of data sets. I have concerns that with an n of 6 compared to 3, some intensities may be made more significant than they are. However, I clearly want to control for machine produced error. Does anyone have any suggestions for dealing with this problem?
Thank you,
Trammells