How to do multiple regression or PCA in MS Dial? October 27, 2022, 12:52:29 PM I am trying to do PCA to find out what compounds are in higher concentrations in one group compared to others and leads to separation. Quote Selected
Re: How to do multiple regression or PCA in MS Dial? Reply #1 – November 02, 2022, 08:33:54 PM PLS-DA may be better for this since it is supervised. Anyway, this can be done by assigning Class ID ['option' tab in toolbar], normalizing (must assign class first; I prefer LOWESS + an internal standard)[data visualization tab], then doing stats in the tool that is also under the data visualization tab (can't remember name off the top of my head).Take care,Triston Quote Selected Last Edit: November 02, 2022, 09:59:59 PM by triston.groff 1 Likes
Re: How to do multiple regression or PCA in MS Dial? Reply #2 – November 07, 2022, 10:04:27 AM Quote from: triston.groff – on November 02, 2022, 08:33:54 PMPLS-DA may be better for this since it is supervised. Anyway, this can be done by assigning Class ID ['option' tab in toolbar], normalizing (must assign class first; I prefer LOWESS + an internal standard)[data visualization tab], then doing stats in the tool that is also under the data visualization tab (can't remember name off the top of my head).Take care,TristonGreat tip, what I struggle to understand is for PLS-DA it requires a y-variable to be defined, would you know how that's done? Quote Selected 1 Likes