Re: Normalization
Reply #5 –
Hi,
simply, I wrote the source code to let you know how the mTIC value is calculated.
Here, LibraryID should be more than 0 if a peak is annotated by MSP library.
The postidentificationlibraryID should be more than 0 if a peak is annotated by user-defined text library containing the pair of m/z and retention time. The annotated metabolite having the tag of "w/o MS2" is excluded to calculate the mTIC value.
Thanks,
Hiroshi
for (int j = 0; j < alignedSpots.Count; j++) {
if ((alignedSpots[j].LibraryID >= 0 && !alignedSpots[j].MetaboliteName.Contains("w/o")) || alignedSpots[j].PostIdentificationLibraryID >= 0)
tic += alignedSpots[j].AlignedPeakPropertyBeanCollection[i].Variable;
}
}