Can't process mzML file in DIA mode using the latest version of MSDIAL October 09, 2020, 07:37:45 AM Dear MSDIAL developers,When using the latest version of MSDIAL (4.24) I received the following error when trying to process an mzML file in DIA mode.QuoteMsdialConsoleApp.exe lcmsdia -i data -o data -m data\Msdial-lcms-dia-Param.txtLoading library files..Start processing..Could not load file or assembly 'MsdialCommon, Version=1.0.0.0, Culture=neutral, PublicKeyToken=null'. The located assembly's manifest definition does not match the assembly reference. (0x80131040)However if I revert to an older version of MSDIAL (4.12) this works:QuoteMsdialConsoleApp.exe lcmsdia -i data -o data -m data\Msdial-lcms-dia-Param.txtLoading library files..Start processing..data\StdMixHigh_DIA1_P1-C-2_01_5747.mzMLend peak pickingExporting peak list data: data\StdMixHigh_DIA1_P1-C-2_01_5747.msdialDeconvoluted peak count: 513data\StdMixHigh_DIA1_P1-C-2_01_5747.mzML finishedFinishedThe params files that I used are attached in this post. I can't include the mzML because it's too big, but I believe this problem happens for all mzML files. I know the documentation says that the DIA mode 'accepts ABF format file only', so I'm very happy to find out that I could use an mzML file directly with an older version of MSDIAL. However it would be good if it works with the latest version too. Quote Selected
Re: Can't process mzML file in DIA mode using the latest version of MSDIAL Reply #1 – October 15, 2020, 04:49:31 AM Hi all,From further testing, it seems that the above works with DDA mode, so the problem only happens for DIA mode. Quote Selected
Re: Can't process mzML file in DIA mode using the latest version of MSDIAL Reply #2 – October 19, 2020, 07:04:03 AM The same problem of not being to load assembly still persists in the latest (v4.36) console version, only for DIA mode. Quote Selected
Re: Can't process mzML file in DIA mode using the latest version of MSDIAL Reply #3 – October 31, 2020, 04:56:46 AM OK, sorry for this late reply. I will address this issue within 2 days.Hiroshi Quote Selected
Re: Can't process mzML file in DIA mode using the latest version of MSDIAL Reply #4 – November 01, 2020, 07:31:09 AM Hi,I fixed this issue and the new version was released today.See: http://www.metabolomics-forum.com/index.php?topic=1576.0Thanks,Hiroshi Quote Selected